Epigenetics Methods

Name: Epigenetics Methods
Price: 250 USD
Availability: InStock
ISBN: 9780443267598

by Trygve O. Tollefsbol

2nd Edition

Publisher: Academic Press

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Book Details

Print ISBN	9780443267598
eText ISBN	9780443267604
Publisher	Academic Press
Publishing Year	2025
Edition	2nd Edition
Language	English
Pages	746

Epigenetics Methods, Second Edition is a reference edited by Trygve O. Tollefsbol and published by Academic Press that provides an overview of analytical tools designed to support translational epigenetics studies. This volume synthesizes complex laboratory protocols into clear, structured procedural guidance for researchers pursuing study in molecular biology.

The material organizes laboratory workflows around primary biological mechanisms of gene regulation. Detailed chapters present validated protocols for assessing DNA methylation, executing histone modification techniques, performing chromatin evaluation, and conducting enzyme analyses. In addition, the text examines non-coding RNAs operating as epigenetic modulators, illustrating how these non-coding transcripts participate in chromatin dynamics and cellular control.

A primary priority of this reference is confronting experimental reproducibility issues that frequently affect studies across different research facilities. By presenting consensus-driven methods to unify experimental approaches across independent laboratories, the volume offers reliable operational frameworks intended to support basic scientists, clinical researchers, and students.

Chapter 1: An Overview of Epigenetics Methods
Chapter 2: Principles of Bisulfite Conversion of DNA and Methylation-specific PCR (MSP) in Biological Research
Chapter 3: Multiplex Methylation Specific Droplet Digital PCR (MS-ddPCR) for Detection and of Rare Methylated Alleles
Chapter 4: Methods for Detection of DNA Methylation in Transposable Elements
Chapter 5: The Illumina Infinium Methylation Assay for Genome-wide Methylation Analyses
Chapter 6: Methylated DNA Immunoprecipitation Sequencing: Methodological Principles and Applications
Chapter 7: Multiplex Analyses Using Methylation Sensitive Restriction Enzyme coupled qPCR and NGS
Chapter 8: Advances in Whole Genome Methylomic Sequencing
Chapter 9: Analyzing DNA Methyltransferase Enzymatic Activity: Basic Methods and Applications
Chapter 10: Technologies and Applications for the Assessment of 5-Hydroxymethylcytosine
Chapter 11: The Chromatin Immunoprecipitation (ChIP) Assay and ChIP-qPCR
Chapter 12: CUT&RUN and CUT&Tag for histone modification and transcription factor binding analyses
Chapter 13: Deep Sequencing for Histone Modifications Employing ChIP-seq Analyses
Chapter 14: ChIP in Series: Sequential ChIP
Chapter 15: The ChIP-exo Method to Study DNA-protein interactions and Chromatin Organization at Near-Nucleotide Resolution
Chapter 16: ATAC-seq Method Applied to Embryonic Germ Cells and Neural Stem Cells from Mouse: Practical
Chapter 17: Combinatorial ChIP and Bisulfite Sequencing (BisChIP-seq)
Chapter 18: 3D DNA FISH for Analyses of Nuclear Chromatin Architecture
Chapter 19: Assessment of Histone-Modifying Enzymes Involved in Epigenetic Gene Regulation
Chapter 20: Detection and Quantification of MicroRNAs (miRNAs) and High Throughput miRNAs Profiling
Chapter 21: RIP-seq in Epigenetic Analyses
Chapter 22: Chromatin Isolation by RNA Purification (ChIRP) and Its Applications
Chapter 23: Single-Cell Epigenomics: Methods and Translation
Chapter 24: CRISPR/Cas9 Technologies in Epigenetics Research
Chapter 25: Epigenome-Wide Association Study (EWAS) Methods and Applications
Chapter 26: Systems Biology Methods to Study the Epigenome and Metabolic- epigenetic Interactions
Chapter 27: Forensic Epigenetics Methods and Applications
Chapter 28: Advances in Epigenetic Techniques to Study Development and Diseases