Next-Generation Sequencing Data Analysis

Next-Generation Sequencing Data Analysis (2nd Edition) provides a clear, structured guide to processing and interpreting modern high-throughput biological data. Published by CRC Press, this textbook equips bioinformaticians, biostatisticians, data scientists, and life sciences practitioners with the analytical methodologies required to direct sequencing projects.

The content outlines a practical end-to-end framework starting with early experimental design, sample processing requirements, and sequencing strategy formulation. Readers progress through early-stage data processing routines, covering read quality control assessment, data preprocessing protocols, and alignment procedures including read mapping or de novo sequence assembly.

To support modern sequencing workflows, the text embeds long-read sequencing coverage across its core analytical sections. By anchoring technical procedures in open-source software tools that are freely available, this resource provides an accessible foundation for upper-level bioinformatics instruction and independent laboratory study.