Building a Cell from Its Component Parts
by Jennifer Ross, Wallace F. Marshall
1st Edition
Publisher: Academic Press
Book Details
| Print ISBN | 9780128024508 |
| eText ISBN | 9780128024508 |
| Publisher | Academic Press |
| Publishing Year | 2015 |
| Edition | 1st Edition |
| Language | English |
Building a Cell from Its Component Parts, 1st Edition, is Volume 128 of the Methods in Cell Biology series from Academic Press. Edited by Jennifer Ross and Wallace F. Marshall, the collection presents experimental methods for assembling cellular structures from basic components.
The text addresses assemblies across multiple spatial scales, spanning individual protein molecules to vesicle- and cell-sized systems. Topics include pairing molecular motors with cytoskeletal elements and integrating those networks with fabricated chambers or pillar arrays.
Organized into sections on model systems, functional studies, imaging-based approaches, and emerging studies, the book serves researchers and students across cell, molecular, and developmental biology.
Table of Contents
Chapter 1: In Vitro Systems for the Study of Microtubule-Based Cell Polarity in Fission Yeast
Chapter 2: Microtubules, MAPs, and Motor Patterns
Chapter 3: Self-Organization of Motors and Microtubules in Lipid-Monolayered Droplets
Chapter 4: Reconstitution of Microtubule Based Motility Using Cell Extracts
Chapter 5: Building Cells for Quantitative, Live-cell Analyses of Collective Motor Protein Functions
Chapter 6: Reconstituting Cytoskeletal Contraction Events with Biomimetic Actin-Myosin Active gels
Chapter 7: Building an Artificial Actin Cortex on Microscopic Pillar Arrays
Chapter 8: Triggering Actin Polymerization in Xenopus Egg Extracts from Phosphoinositide-Containing Lipid Bilayers
Chapter 9: Reconstituting Geometry Modulated Protein Patterns in Membrane Compartments
Chapter 10: Structural and Functional Studies of Membrane Remodeling Machines
Chapter 11: Building Interconnected Membrane Networks
Chapter 12: Using Supported Bilayers to Study the Spatiotemporal Organization of Membrane Bound Proteins
Chapter 13: Reconstituting ParA/ParB-Mediated Transport of DNA Cargo
Chapter 14: Cell-Sized Liposomes that Mimic Cell Motility and the Cell Cortex
Chapter 15: Reconstitution of Cortical Actin Networks Within Water-In-Oil Emulsions
Chapter 16: Engineering Artificial Cells by Combining HeLa-Based Cell-Free Expression and Ultra-Thin Double Emulsion Template
Chapter 17: Reconstitution of Proteins on Electroformed Giant Unilamellar Vesicles
Chapter 18: Reconstituting SNARE-Mediated Membrane Fusion at the Single Liposome Level
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