Protocols in Biochemistry and Clinical Biochemistry

Name: Protocols in Biochemistry and Clinical Biochemistry
Price: 175 USD
Availability: InStock
ISBN: 9780443139451

by Buddhi Prakash Jain, Shweta Pandey, Shyamal K Goswami

2nd Edition

Publisher: Academic Press

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Book Details

Print ISBN	9780443139451
eText ISBN	9780443139468
Publisher	Academic Press
Publishing Year	2024
Edition	2nd Edition
Language	English
Pages	237

Protocols in Biochemistry and Clinical Biochemistry, 2nd Edition, provides a comprehensive compilation of laboratory methods designed for fundamental benchwork and clinical diagnostics. Authored by Buddhi Prakash Jain, Shweta Pandey, and Shyamal K Goswami, the book guides students, clinicians, and active researchers working across biochemistry, molecular biology, and cell biology. It establishes clear operational directions for conducting precise chemical assays and physiological evaluations.

The technical content spans several essential analytical domain arcs, moving from primary solution preparation to complex metabolic testing. The initial sections cover buffer preparation, nucleic acid purification, and detailed testing procedures for carbohydrates, lipids, proteins, and enzymes. Later sections shift toward clinical diagnostic applications, outlining exact quantitative procedures to measure parameters such as vitamin concentrations, glucose levels, and total serum cholesterol.

Each of the 100 protocols features step-by-step instructions, labeled diagrams, color images, worked examples, and clear key term definitions. This uniform structure helps laboratory practitioners execute procedures correctly while mastering essential analytical techniques. The text accommodates both introductory learning and advanced experimental execution within academic and medical research settings.

Chapter 1: Preparation of Solutions
Chapter 2: Acid and Base
Chapter 3: Determination of the presence of carbohydrates in the given sample by Molisch’s test
Chapter 4: Determination of the presence of reducing carbohydrates in the given sample by Picric acid test
Chapter 5: Determination of the presence of reducing carbohydrates in the given sample by Fehling’s test
Chapter 6: Determination of the presence of reducing carbohydrates in the given sample by Benedict’s test
Chapter 7: Determination of the presence of reducing carbohydrates in the given sample by Tommer’s test
Chapter 8: Determination of the presence of reducing carbohydrates in the given sample by Nylander’s test
Chapter 9: Distinguish between monosaccharides and reducing disaccharides by Barfoed’s test.
Chapter 10: Distinguish between aldose and ketose by Seliwanoff’s test.
Chapter 11: Determination of pentose sugar by Bial’s test
Chapter 12: Determination of the presence of galactose in a test sample by the Mucic acid test
Chapter 13: Differentiation between the presence of ketohexose or aldohexose in the test sample by Foulger'stest
Chapter 14: Determination of the presence of reducing carbohydrates in the given sample by Fearon’s methylamine test
Chapter 15: Confirmation of the presence of reducing carbohydrates in the given sample by osazone formation test
Chapter 16: Determination of the presence of polysaccharides in the given sample by an iodine test
Chapter 17: Quantitative estimation of carbohydrates in the given sample by anthrone test.
Chapter 18: Quantitative estimation of reducing carbohydrates in the given sample by 3,5-dinitro salicylic acid (DNSA) test.
Chapter 19: Extraction and estimation of glycogen from the liver and muscle of the well-fed and starved rat.
Chapter 20: Separation and Identification of sugars present in fruit juices using thin-layer chromatography (TLC).
Chapter 21: Extraction and analysis of soluble carbohydrates from plants.
Chapter 22: Solubility test for lipids
Chapter 23: Emulsion test for lipids.
Chapter 24: To determine the degree of unsaturation of fatty acids
Chapter 25: Determination of the saponification value of the given fat/ oil sample
Chapter 26: To determine the fatty acid value of fat
Chapter 27: Acrolein test for the presence of glycerol
Chapter 28: Libermann – Buchard test for detection of cholesterol.
Chapter 29: Extraction of lipid from leaves (Bligh and Dyer’s method and single extraction method).
Chapter 30: Extraction of lipid from egg yolk and estimation of phosphorous content in it
Chapter 31: Extraction of lipid from tissues (Folch method).
Chapter 32: Identification of lipids by TLC.
Chapter 33: Lipid per oxidation measurement by Thiobarbituric acid (TBA) assay.
Chapter 34: Isolation of protein from animal cells/tissue using RIPA buffer
Chapter 35: Qualitative test for Proteins and amino acids
Chapter 36: Estimation of proteins by Biuret method
Chapter 37: Estimation of proteins by Bradford method
Chapter 38: Estimation of proteins by Bicinchoninic acid (BCA) method
Chapter 39: Estimation of proteins by Folin-Lowry method
Chapter 40: Estimation of proteins by UV spectrophotometric method
Chapter 41: Protein separation by gel electrophoresis under denaturing condition (SDS-PAGE)
Chapter 42: Separation of amino acids by paper chromatography.
Chapter 43: Estimation of the Calcium in water or milk by EDTA titrimetric method
Chapter 44: Estimation of the Calcium in serum by O-Cresolpthaleincomplexon method (colorimetric)
Chapter 45: Estimation of the inorganic phosphorus by colorimetric method
Chapter 46: Estimation of the Iron by thiocyanate colorimetric method
Chapter 47: Estimation of the Chloride by Titration method (Mohr Argentometric method)
Chapter 48: Estimation of Vitamin A by Trichloroacetic acid (modified Carr-Price method)
Chapter 49: Estimation of the Tocopherol (Vitamin E) by colorimeter (Baker and Frank method)
Chapter 50: Estimation of the Thiamine (Vitamin B1) by Thiochrome technique
Chapter 51: Estimation of the Ascorbic acid (Vitamin C) by 2,6-dichlorophenol indophenol (DCPIP) method (Titrimetric)
Chapter 52: Estimation of the Ascorbic acid (Vitamin C) by 2, 4 Dinitrophenylhydrazine method (Colorimetric)
Chapter 53: To study the effect of pH and temperature on the action of salivary amylase.
Chapter 54: Determination of the activity of amylase enzyme (crude) extracted from sweet potato.
Chapter 55: Determination of the specific activity of the amylase enzyme.
Chapter 56: To determine Vmax and Km in of the amylase enzyme.
Chapter 57: Determination of the amount of DNA present in the solution by diphenylamine (DPA) method
Chapter 58: Determination of the amount of RNA present in the solution by orcinol method.
Chapter 59: Isolation of plasmid DNA using the alkaline lysis method.
Chapter 60: Preparation of plasmid DNA using boiling lysis method.
Chapter 61: Plasmid DNA purification using polyethylene glycol.
Chapter 62: Isolation of genomic DNA of the unknown bacterial strain.
Chapter 63: Isolation of DNA from plant tissue.
Chapter 64: Isolation of DNA from a eukaryotic cell using proteinase K and phenol.
Chapter 65: Isolation of DNA from a eukaryotic cell.
Chapter 66: Separation of bands of DNA by agarose gel electrophoresis.
Chapter 67: SOUTHERN TRANSFER AND HYBRIDIZATION: To perform Southern blotting of the genomic DNA.
Chapter 68: Isolation of RNA from animal cells.
Chapter 69: NORTHERN BLOTTING.
Chapter 70: Determination of Serum Urea [Diacetyl Monoxime (DAM) Method],
Chapter 71: Estimation of Uric Acid in Serum Sample (Uricase Method),
Chapter 72: Estimation of Creatinine in Serum Samples (Jaffe’s Method),
Chapter 73: Determination of Serum Glutamate Pyruvate Transaminase (SGPT) Activity,
Chapter 74: Determination of Serum Glutamate Oxaloacetate Transaminase (SGOT) Activity,
Chapter 75: Colorimetric Estimation of Alkaline Phosphatase (ALP) From Serum (King and Armstrong),
Chapter 76: Estimation of Serum Albumin by the Dye-Binding Method (Bromocresol Green Method),
Chapter 77: Estimation of Blood Glucose by the Glucose Oxidase Method,
Chapter 78: Estimation of Glucose in Urine by Benedict Method,
Chapter 79: Estimation of Percentage of Glucose (Reducing Sugars) in Urine (by Benedict Titration Method),
Chapter 80: Oral Glucose Tolerant Test (OGTT),
Chapter 81: Estimation of Total Cholesterol in Serum (Watson Method),
Chapter 82: Estimation of Total Cholesterol in Serum (Enzymatic Method),
Chapter 83: Estimation of Triglycerides in Serum (Enzymatic Method),
Chapter 84: Estimation of Hemoglobin in the Blood (Sahli’s Method).
Chapter 85: Determination of serum amylase.
Chapter 86: Catalase activity assay.
Chapter 87: Superoxide dismutase (SOD) assay.
Chapter 88: Qualitative analysis of plant secondary metabolites.
Chapter 89: Qualitative analysis of photochemical.
Chapter 90: Quantitative estimation of secondary metabolites.
Chapter 91: Estimation of Chlorophyll contents.
Chapter 92: Determination of starch